Talk given by Roland Drexel at the virtual NanoSafety 2020 conference highlighting the benefits of Electrical and Asymmetrical Flow Field-Flow Fractionation hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis for the analysis of exosomes and liposomes in complex biological media. The successful hyphenation of FFF and NTA is one of the major outcomes of the collaboration of Postnova Analytics with Malvern Panaalytical within the frame of the Horizon 2020 project ACEnano /http://www.acenano-project.eu/).