PN3460 Raman Detector

Raman Scattering has become more and more popular as an information rich spectroscopy method additional to RI, UV and Fluorescence based techniques. Also in nano, bio and polymer science it has proved it applicability to a wide range of applications. Raman microscope as a laser-powered microscopic device is an very efficient way to perform Raman spectroscopy.

Typical Raman microscopes begin with a standard optical microscope by adding a specific excitation laser, laser rejection filters, either a spectrometer or a monochromator and optical sensitive CCD or PMT detectors.

One way to use Raman microscopy is by measuring the Raman spectrum of a single point. However, recently the technique has been extended to apply Raman spectroscopy for direct chemical imaging over the whole field of view on a 2D/3D sample. Raman microscopy can reach down to sub-micrometer lateral spatial resolution and when used with a Postnova flow cell, allows detection and characterization of nano and micro particles separated by the Postnova FFF platform beforehand.

The Postnova PN3460 Raman Flow Cell enables the online hyphenation of all liquid sample systems and especially Field-Flow Fractionation combined with Raman microscopy for highresolution separation and chemical identification of particles in the size range of 200 nm up to 50 μm. It is particularly useful for the analysis of micro- and nanoplastic particles.

The Postnova PN3460 Raman Flow Cell has been validated with the WITec alpha300 access confocal Raman microscope.
Recommended Objective:
Olympus Water Immersions Objective LUMPLFLN40XW (W.D. 3.3, Mag 40, OFN 26.5, NA 0.8, IM water).
Any other comparable lens would also be usable.